Kinetic mechanisms for the sequence dependence of transcriptional errors.

ABSTRACT: The fidelity of template-dependent mRNA synthesis during transcription elongation is the primary determinant of accurate gene expression and the maintenance of functional RNA transcripts. However, the mechanisms governing transcription fidelity remain incompletely understood. While previous studies have characterized how error rates vary with nucleotide identity at upstream and downstream positions from the incorporation site, the comprehensive microscopic explanation of this sequence dependence has not been elucidated. In this study, we develop a theoretical approach that integrates transcription proofreading mechanisms and inhomogeneous DNA sequence effects. Using first-passage analysis validated by Monte Carlo simulations, we quantitatively characterize nucleotide-specific error rates d

SUBMITTER: Midha T 

PROVIDER: S-EPMC12280891 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature