Electrochemically controlled switching of dyes for enhanced superresolution optical fluctuation imaging (EC-SOFI).

ABSTRACT: In superresolution optical fluctuation imaging (SOFI), molecules spaced closer than the diffraction limit can be separated through spatial and temporal correlation analysis of the fluorescence intensity fluctuations. The resolution and speed of SOFI imaging greatly depend on the characteristics of these fluorescence fluctuations. Fluorophores with stochastic and rapid fluorescence fluctuations are favorable for improving SOFI imaging resolution and speed, especially in high-order cumulant analysis. Stochastic blinking of organic dyes in oxygen scavenging and thiol reducing buffers are well suited for SOFI due to their high brightness. However, the blinking rates can be too slow and nonuniform, which introduces nonlinearity in SOFI image. To address these challenges, we introduce electroche

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12280914 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature