A standardized soil-based biotest to investigate the phytoavailability of nanoplastics.

ABSTRACT: Standardized methods are essential for generating reliable and reproducible data to support risk assessment and decision-making related to soil contamination by environmental pollutants, including nanoplastics (NPs). This study evaluated the ability of the RHIZOtest method, a standardized soil-plant exposure system, in providing a high-throughput testing platform for investigating NP phytoavailability. As a proof of concept, tomato plants were exposed to artificial soil spiked with model NPs at concentrations of 400 and 4000 mg kg^-1 dm. Palladium (Pd)-doped polystyrene particles (PS-P) (a Z-average diameter of 210 nm, a surface charge zeta potential of -45.20 ± 032 mV, a polydispersity index of 0.1, and a Pd doping ratio of 0.295% w/w Pd to PS-P) were used as surrogates for NPs.

SUBMITTER: Beggio G 

PROVIDER: S-EPMC12281096 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature