Chromatin accessibility dynamics and transcriptional regulatory networks underlying the primary nitrogen response in rice roots.

ABSTRACT: Nitrogen (N) is essential for rice growth; however, the transcriptional regulation of the primary nitrogen response (PNR), characterized by the rapid upregulation of N uptake and assimilation genes upon N resupply, remains poorly understood. This study investigated the dynamics of the PNR in the roots of two rice cultivars (Zhenshan 97 and Nipponbare) via time-series Assay for Transposase-Accessible Chromatin using sequencing and RNA sequencing analyses within 2 h of ammonium nitrate resupply. Regulatory regions responsive to N induction were precisely identified. Coordinated and cascading changes in chromatin accessibility and gene expression were observed, with chromatin state frequently preceding transcriptional changes. Integrative analysis of expression-chromatin accessibility associa

SUBMITTER: Li W 

PROVIDER: S-EPMC12281222 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature