Quantum Dot-Based Immunolabelling of Extracellular Vesicles and Detection Using Fluorescence-Based Nanoparticle Tracking Analysis.

ABSTRACT: Extracellular vesicles (EVs) contain a variety of biomolecules, including DNA, RNA, lipids and proteins. They can interact with target cells to perform various functions, offering potential for therapeutic applications like drug delivery and diagnosis. The growing interest in EVs drives the need for robust methods for EV characterisation. One of the prevalent EV characterisation methods is scatter-based nanoparticle tracking analysis (Sc-NTA). This method measures the size and concentration of particles by tracking the scattered light from individual particles. However, Sc-NTA has limitations in selectivity, as it detects all scattered light and fails to distinguish EVs from other nanoparticles, such as protein aggregates. To overcome this limitation, fluorescence-based NTA (Fl-NTA) is bei

SUBMITTER: Ha E 

PROVIDER: S-EPMC12281464 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature