Arabinokinase Limits the Flux of Arabinose Into Nucleotide Sugars to Prevent Toxicity.

ABSTRACT: Arabinokinase (ARA1) is a key player in the recycling pathway of the major cell wall component L-arabinose (L-Ara). The enzyme catalyzes phosphorylation of L-Ara to L-arabinose-1-phosphate, which is then converted into UDP-L-arabinopyranose (UDP-L-Arap) by UDP-sugar pyrophosphorylase (USP) followed by conversion into UDP-L-arabinofuranose (UDP-L-Araf) by UDP-arabinopyranose mutases (UAM) before it is incorporated into cell wall polymers. While this pathway is typically nonessential for plant development, a threefold accumulation of UDP-L-Arap can lead to toxicity. To investigate this, we generated Arabidopsis thaliana lines overexpressing the kinase domain of ARA1 (ARAK1-OE) and examined their response to L-Ara feeding. ARAK1-OE seedlings revealed dose-dependent

SUBMITTER: Kavkova EI 

PROVIDER: S-EPMC12281597 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature