Establishing a Malonyl-CoA Biosensor for the Two Model Cyanobacteria <i>Synechocystis</i> sp. PCC 6803 and <i>Synechococcus elongatus</i> PCC 7942.

ABSTRACT: Malonyl-CoA, produced by the first committed step of fatty acid biosynthesis, is a precursor for many valuable bioproducts, making it an important metabolic engineering target. Here, we establish a malonyl-CoA biosensor for the model cyanobacteria Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. The developed biosensor utilizes FapR, a malonyl-CoA-regulated transcriptional repressor from Bacillus subtilis, and novel FapR-regulated and cyanobacteria-compatible hybrid promoters for expressing Yfp, the biosensor output reporter. A l-rhamnose-inducible promoter P_rhaBAD, characterized in combination with ribosome binding sites of varied strengths, was evaluated for titratable FapR expression. Additionally, the placement and quantity

SUBMITTER: Cengic I 

PROVIDER: S-EPMC12281612 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature