Illuminating Glucomannan Synthases To Explore Cell Wall Synthesis Bottlenecks.

ABSTRACT: Hemicelluloses are important dietary fibers and a key component of lignocellulosic biomass. Despite numerous observations for fluorescently tagged cellulose synthases, the subcellular journeys and biochemical activities of intracellular cellulose synthase-like enzymes such as β-mannan synthases (ManS) remain largely unexplored. This study identifies C-terminal fluorescent protein tags that maintain ManS activity in yeast to accelerate the Design, Build, Test, Learn cycles for polysaccharide biosynthesis. Using the Amorphophallus konjac ManS as a case study, we demonstrate that the enzyme colocalizes with a known yeast marker for the Golgi apparatus despite the toxic effects of plant glucomannan accumulation in Pichia pastoris. The ManS first transmembrane domain was found to

SUBMITTER: Grieß-Osowski A 

PROVIDER: S-EPMC12281619 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature