Botulinum toxin type A inhibits microglia pyroptosis by suppressing Cblb-mediated degradation of Pdlim1 to attenuate neuropathic pain.

ABSTRACT:

Background

Microglia pyroptosis, a newly identified form of inflammatory cell death, is involved in the development of neuropathic pain (NP). Botulinum toxin type A (BTX-A) has been shown to be effective in relieving NP, but the mechanisms involved have not been clarified.

Methods

A mice model of NP was established with chronic constriction injury (CCI) method. The expression levels of key molecules and the extent of microglia pyroptosis were assessed using RT-qPCR, western blot, ELISA and immunofluorescence. Moreover, lipopolysaccharide (LPS) was used in vitro to induce pyroptosis of microglia to explore the potential molecular mechanisms of BTX-A.

Result

In a mice model of NP, BTX-A administration increased the pain threshold and decreased the Cblb protein expressio

SUBMITTER: Tian S 

PROVIDER: S-EPMC12281680 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature