Integrated physiological, biochemical and transcriptomic analyses elucidate the response of alpine plant Lamiophlomis rotata (Benth.) Kudo to Low-Nitrogen stress.

ABSTRACT: Lamiophlomis rotata (Benth.) Kudo, an endangered medicinal plant endemic to the Qinghai-Tibet Plateau (altitude of 3100-5000 m), faces conservation challenges due to wild resource scarcity and cultivation difficulties. This investigation employed a gradient nitrogen stress system (N1-N4) with a nitrogen-free control (CK) to decipher its nitrogen adaptation mechanisms. Key findings revealed: (1) superior biomass accumulation, SPAD content, and total nitrogen levels in N1 compared to N4; (2) N4-induced suppression of antioxidant enzymes concurrent with elevated secondary metabolites; (3) transcriptomic perturbations in nitrogen metabolism, monoterpenoid biosynthesis, and photosynthetic pathways; (4) identification of eight nitrogen metabolism-related genes and eight iridoid synthase-associat

SUBMITTER: Zhong L 

PROVIDER: S-EPMC12281743 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature