Pidotimod alleviated experimental autoimmune encephalomyelitis by regulating the balance of splenic lymphocytes.

ABSTRACT:

Objective

To examine whether pidotimod affects the progression and severity of experimental autoimmune encephalomyelitis (EAE), a classic animal model of multiple sclerosis (MS), the balance of splenic lymphocytes in pidotimod-treated and untreated EAE mice was examined.

Methods

C57BL/6J mice were immunized by subcutaneous injection of an emulsion containing MOG35-55, with subsequent monitoring of their general condition and clinical scores following treatment with pidotimod or saline solution (vehicle control). Hematoxylin and eosin (H&E) staining, along with flow cytometry (FCM), was employed to evaluate leukocyte infiltration, while FluoroMyelin™ Green staining was utilized to assess axonal demyelination in the central nervous system (CNS). Additionally, FCM was conducted

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12281762 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature