Reverse genetics rescue of sylvatic dengue viruses.

ABSTRACT: There are an unknown number of sylvatic ("sylvan" = "of the forest") dengue viruses currently being sustained by nonhuman primates and mosquitoes in the forests of Africa and Asia. Humans are regularly infected with these viruses, occasionally resulting in small epidemics. One barrier to understanding sylvatic dengue virus biology is the scarcity of stocks available for study. While the full-length genome sequences of 28 sylvatic viruses exist on GenBank, accessible infectious stocks exist for only a handful of these. One way to overcome this obstacle is to rescue infectious virus stocks using reverse genetics. However, reverse genetic engineering of dengue viruses is notoriously difficult. Here, we optimize a reverse genetics method specifically for the rescue of sylvatic dengue virus sto

SUBMITTER: Gendler P 

PROVIDER: S-EPMC12282064 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature