Late gene regulation by the alternative sigma factors of <i>Chlamydia trachomatis</i>.

ABSTRACT: The pathogenic bacterium Chlamydia reproduces via two specialized forms inside a eukaryotic host cell. The dividing form called the reticulate body (RB) must convert at late times into the infectious elementary body (EB) for spread to new host cells. Late genes are a temporal class of chlamydial genes believed to be responsible for RB-to-EB conversion, but late gene regulation is incompletely understood. In this study, we used chromatin immunoprecipitation (ChIP) to investigate two alternative sigma factors, σ²⁸ and σ⁵⁴, that alter the promoter specificity of Chlamydia trachomatis RNA polymerase. σ²⁸ ChIP-seq identified hctB and tsp as the only promoters bound by σ²⁸, and binding only occurred late, around the time of

SUBMITTER: Rizvi SMA 

PROVIDER: S-EPMC12282069 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature