Engineering a recombination-resistant live attenuated vaccine candidate with suppressed interferon antagonists for PEDV.

ABSTRACT: Porcine epidemic diarrhea virus (PEDV) is a deadly coronavirus (CoV) for neonatal piglets, and no effective vaccine is available. Live attenuated vaccines (LAVs) show promise, but risks of recombination and reversion to virulence hinder their application. For LAV development, we previously engineered a recombination-resistant PEDV mutant RMT by rewiring the transcriptional regulatory sequence (TRS)-core sequences (TRS-CSs). Because TRS is critical for CoV replication, the incompatibility between wild-type TRS-CS and the rewired TRS-CS should disrupt the structural and accessory protein-encoding mRNA transcription from a recombinant PEDV genome, preventing the production of progeny infectious viruses. However, the RMT contained a 189-nt insertion upstream of the E gene TRS-CS and a missing

SUBMITTER: Liu M 

PROVIDER: S-EPMC12282079 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature