Kaposi's sarcoma-associated herpesvirus ORF61 protein sequesters APOBEC3B in filamentous aggregates.

ABSTRACT: Herpesviruses are large DNA viruses that encode homologs of cellular enzymes. The viral ribonucleotide reductase, which consists of large R1 and small R2 subunits, is required for deoxyribonucleotide synthesis. However, herpesviruses have repurposed the R1 subunit for additional non-canonical functions in virus-host interaction and immune evasion. Here, we investigated the R1 proteins of Kaposi's sarcoma-associated herpesvirus (KSHV) and murine gammaherpesvirus 68 (MHV-68), two γ-herpesviruses of the genus Rhadinovirus. We show that the ORF61-encoded viral R1 proteins form elongated cytoplasmic condensates in infected cells, which structurally differ from the previously described R1 condensates of other herpesviruses. Fluorescently labeled ORF61 condensates exhibited the properties

SUBMITTER: Luoto L-M 

PROVIDER: S-EPMC12282084 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature