Trade-off between resistance and persistence in high cell density cultures.

ABSTRACT: Bacterial growth to high cell densities is commonly observed in infections, microbiomes, and biomanufacturing, yet there is limited understanding of how transcriptional regulation changes to balance between growth and survival under associated stresses. Here, we utilized well-controlled culturing systems to grow wild-type and metabolically engineered Escherichia coli strains into high cell densities (50-80 g_{cell dry weight} L^-1) and determine the associated transcriptional dynamics. Knowledge-enriched, machine-learning-based data analytics reveal distinct stress-related gene expression patterns that are consistent with a fundamental trade-off between resistance and persistence. We suggest that this trade-off explains observed growth arrests in high-density cultu

SUBMITTER: Beulig F 

PROVIDER: S-EPMC12282090 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature