Multi-omics characterization of a lytic phage targeting <i>Listeria monocytogenes</i>.

ABSTRACT: Listeria monocytogenes is a foodborne pathogenic bacterium that can persist in food-processing environments. Strictly lytic Listeria phages have shown promise as biosanitation and biocontrol agents. However, little is known about the molecular progression of phage expression and the host gene expression profile it elicits in Listeria. In this work, the P100-like phage CKA15 was characterized using a proteogenomics-based approach to identify virion-associated proteins, Illumina-based RNA-seq to analyze time-resolved host and phage transcript abundance during infection, and ONT-cappable-seq to experimentally determine the operon structure of the phage genome. We detected 29 phage-encoded putative particle-associated proteins. During infection, a progressive decrease in h

SUBMITTER: Cucic S 

PROVIDER: S-EPMC12282133 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature