Multi-level regulation of hindgut homeostasis by volatile fatty acid administration in dairy goats: linking microbial metabolism to epithelial inflammation and barrier function.

ABSTRACT: This study provided a comprehensive exploration of the nutritional regulation of volatile fatty acids (VFAs) on hindgut microbial metabolism and epithelial homeostasis in dairy goats. Twenty-four goats were orally administered sodium acetate (SA) at 0.8 g/kg of body weight (BW), propionate (SP) at 0.8 g/kg of BW, butyrate (SB) at 0.5 g/kg of BW, or saline (CON) before morning feeding for 12 days (n = 6/group). Serum and hindgut epithelial tissues were collected to measure antioxidant capacity, inflammatory cytokines, and tight junctions. Cecal contents were collected for 16S rRNA sequencing and metabolome analysis, and colonic epithelial cells were harvested for transcriptome sequencing. The data demonstrated that VFAs positively affected hindgut homeostasis. SB reduced serum malond

SUBMITTER: Zhen Y 

PROVIDER: S-EPMC12282142 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature