Impact of the changes in substrate specificity of herpes simplex virus 1 protein kinase Us3 on viral infection <i>in vitro</i> and <i>in vivo</i>.

ABSTRACT: A serine-threonine protein kinase (PK), Us3, encoded by herpes simplex virus 1 (HSV-1), shares substrate specificity with host cellular PKs, protein kinase A (PKA), and AKT. Many Us3 substrates have been identified, and it is thought that during HSV-1 infection, Us3 fine-tunes the phosphorylation levels of individual substrates within their repertoire. However, the significance of this regulatory fine-tuning by Us3 during HSV-1 infection is poorly understood. Here, we found alanine at position 326 (Ala-326) in Us3 was required for the proper fine-tuning of Us3-mediated phosphorylation across the target repertoire in HSV-1-infected cells. Using recombinant viruses in which Us3 Ala-326 was replaced with valine (A326V) or isoleucine (A326I), we showed these mutations selectively altered the p

SUBMITTER: Shio S 

PROVIDER: S-EPMC12282182 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature