Development of a new N-terminomic method to study the pathodegradome of the <i>Staphylococcus aureus</i> V8 protease in human neutrophils.

ABSTRACT: Staphylococcus aureus is a notorious human pathogen that relies on an array of virulence factors to engender infection and evade the host-immune system. Among these are the secreted proteases, which promote pathogenesis by degrading host proteins and modulating host defenses. Human neutrophils play a pivotal role in these defenses, acting as the first responders against invading bacteria. While many S. aureus effectors of virulence have been shown to target leukocytes, there is limited knowledge on how the extracellular proteases modulate neutrophil fate. Typically, protease substrates have been identified in isolated settings using one-at-a-time approaches, with neutrophil targets few and far between. Herein, we have developed a novel N-terminomic methodology termed TAGS-CR

SUBMITTER: Mustor EM 

PROVIDER: S-EPMC12282195 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature