Evaluating methods for genome sequencing of <i>Chlamydia trachomatis</i> and other sexually transmitted bacteria directly from clinical swabs.

ABSTRACT: Rates of bacterial sexually transmitted infections (STIs) are rising, and accessing their genomes provides information on strain evolution, circulating strains and encoded antimicrobial resistance (AMR). Notable pathogens include Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Treponema pallidum (TP), globally the most common bacterial STIs. Mycoplasmoides (formerly Mycoplasma) genitalium (MG) is also a bacterial STI that is of concern due to AMR development. These bacteria are also fastidious or hard to culture, and standard sampling methods lyse bacteria, completely preventing pathogen culture. Clinical samples contain large amounts of human and other microbiota DNA. These factors hinder the sequencing of bacterial STI genomes. We aime

SUBMITTER: Buttner KA 

PROVIDER: S-EPMC12282229 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature