Bioinformatic discovery of type 11 secretion system (T11SS) cargo across the <i>Proteobacteria</i>.

ABSTRACT: Type 11 secretion systems (T11SS) are broadly distributed amongst Proteobacteria, with more than 3,000 T11SS family outer membrane proteins (OMPs) comprising ten major sequence similarity network clusters. Of these, only seven, all from animal-associated cluster 1, have been experimentally verified as secretins of cargo, including adhesins, haemophores and metal-binding proteins. To identify novel cargo of a more diverse set of T11SS, we identified gene families co-occurring in gene neighbourhoods with either cluster 1 or marine microbe-associated cluster 3 T11SS OMP genes. We developed bioinformatic controls to ensure that perceived co-occurrences are specific to T11SS, and not general to OMPs. We found that both cluster 1 and cluster 3 T11SS OMPs frequently co-occur with single-ca

SUBMITTER: Grossman AS 

PROVIDER: S-EPMC12282271 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature