Linking volatile metabolites from bacterial pathogens to exhaled breath condensate of people with cystic fibrosis.

ABSTRACT: Obtaining sputum samples from people with cystic fibrosis (pwCF) for microbiology has become challenging due to the positive clinical effects of the cystic fibrosis transmembrane conductance regulator modulator therapy, elexacaftor-tezacaftor-ivacaftor (ETI). Although ETI improves lung function and reduces sputum production, recent data shows that bacterial pathogens persist, making continued monitoring of infection important. As an alternative to sputum sampling, this study developed a non-invasive technique called 'Cough Breath' (CB) to identify volatile organic compounds (VOCs) in exhaled breath condensate (EBC) and link them to cystic fibrosis (CF) bacterial pathogens using purge and trap GC-MS. The CB culturing approach was able to isolate pathogens from expectorated particulates simu

SUBMITTER: Karunarathne PH 

PROVIDER: S-EPMC12282304 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature