Improved efficiency of two-step amplicon PCR using an acoustic liquid handler.

ABSTRACT: The improvement in next-generation sequencing technologies has reduced the costs of sequencing significantly. However, library preparation costs for amplicon sequencing have remained largely unchanged - which is ultimately the cost-limiting step in processing large numbers of microbiome samples. Acoustic liquid handlers can transfer volumes as low as 2.5 nl and have been used to miniaturize several different molecular and cellular assays, including single-step PCR amplicon library preparations. However, there are no current methods available for a two-step library preparation process using an acoustic liquid handler. In this study, we tested the efficiency of an acoustic liquid handler to automate the PCRs and library quantification while also incorporating automated library bead cleanup.

SUBMITTER: Benz BR 

PROVIDER: S-EPMC12283028 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature