Highly Conserved Genetic Factors Regulating <i>bla</i>_NDM Gene Expression.

ABSTRACT: The New Delhi metallo-β-lactamase (NDM; EC 3.5.2.6) poses significant challenges to carbapenem treatment, yet the regulatory mechanisms governing bla_NDM gene expression remain poorly understood. In this study, we identified a highly conserved 110 bp sequence located upstream of the bla_NDM gene by comparative analysis of 109 clinical isolates and 2,706 nucleotide sequences from the NCBI database. This conserved sequence was characterized in all available NDM variants spanning 80 bacterial species. Bioinformatics analysis revealed a core promoter (PNDM) and two transcription factor binding sites (ArcA and ArgR2) within the sequence. Mutations of the PNDM promoter significantly reduced bla_NDM expression (mRNA and protein) by more th

SUBMITTER: Xu J 

PROVIDER: S-EPMC12283253 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature