The use of three-dimensional primary human myospheres to explore skeletal muscle effects of in vivo krill oil supplementation.

ABSTRACT:

Purpose

Supplementation with krill oil has shown effects on whole-body lipid and glucose metabolism, as well as on skeletal muscle strength and function. We previously showed that krill oil intervention in vivo promoted fatty acid metabolism and protein synthesis in cultured human myotubes in a two-dimensional (2D) model. The aim of this study was to explore the effects of krill oil supplementation in vivo in a 3D myosphere model, and to compare a the human skeletal muscle 3D cell model to a 2D model.

Methods

Myospheres were formed from myoblasts obtained before and after 7 weeks of in vivo krill oil intervention. Glucose and oleic acid metabolism were assessed, and transcriptomic and proteomic analyses were performed.

Results

In vivo intervention with krill oil incre

SUBMITTER: Dalmao-Fernandez A 

PROVIDER: S-EPMC12283505 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature