In vitro model assesses the susceptibility of polymeric scaffolds for material-driven heart valve regeneration to calcification.

ABSTRACT:

Purpose

Material driven in situ heart valve tissue engineering (HVTE) prospects an alternative to non-living replacements. HVTE exploits bioresorbable (synthetic) scaffolds that guide neo-tissue formation. Proper scaffold design assesses and mitigates potential material-related risks, such as calcific nodule formation. Herein, we establish an in vitro model to investigate the calcification risk of materials for HVTE.

Methods

Calcification was studied by culturing 3D scaffolds with porcine valvular interstitial cells in a phosphate-enhanced calcification medium (CM) for 3 weeks. The model was applied by testing three electrospun polymeric Tissue engineering (TE) scaffolds (PCL, PCL-BU, and PC-BU) against a bovine pericardial patch control. Additionally, the model included a 1

SUBMITTER: van der Valk DC 

PROVIDER: S-EPMC12283539 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature