The development of a direct co-culture-based model for diabetic foot ulcer mimicking inflammation and impaired phagocytosis.

ABSTRACT:

Purpose

Diabetic foot ulcers (DFU) are characterized by delayed healing and high infection rates. DFU affect approximately 25% of individuals with diabetes. Secondary to hyperglycaemia, both chronic inflammation and defective phagocytosis have been identified as contributing factors to the non-healing status of DFU. Both inflammation and defective phagocytosis in DFU were sought to be modelled in vitro using pHRODO bioparticles for the first time. The pHRODO bioparticles, popularly used as phagocytic cargos, are chemically killed microorganisms conjugated to the pH-sensitive pHRODO dye that solely fluoresces within the acidic lysosomes where phagocytosis occurs.

Methods

The in vitro DFU model was developed by identifying which ratio of diabetic fibroblasts to THP-1-derived M

SUBMITTER: Ejiugwo M 

PROVIDER: S-EPMC12283541 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature