Efficient expression of a novel <i>α</i>-amylase for reduction of tobacco starch and smoke hazard.

ABSTRACT: The combustion of excessive starch in tobacco leaves leads to more harmful substances, adversely affecting the sensory properties of tobacco and posing significant risks to human health. Therefore, there is an urgent need to develop specific amylases targeting tobacco starch to address these issues. In this study, 5 different α-amylase genes were selected for recombinant expression in Bacillus amyloliquefaciens BAX-5, and the amyA(LC) (derived from Bacillus amyloliquefaciens MK10163) was confirmed to be the optimal gene. Then, the α-amylase activity was further increased by screening host bacteria BAX-5 and signal peptides SP₀₀₃ (derived from the dacB gene of Bacillus subtilis 168). Subsequently, the α-amylase properties we

SUBMITTER: Han Z 

PROVIDER: S-EPMC12283668 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature