STAT3 mediates CAF-induced osimertinib resistance via regulating protein secretion in non-small cell lung cancer.

ABSTRACT:

Introduction

EGFR-TKI resistance is an important factor limiting the clinical application of targeted drugs in NSCLC, but the mechanism remains unclear. The tumor microenvironment is the internal environment for cancer cells to survive, and it plays an important role in tumor resistance.

Methods

In vitro assays: CCK-8 assay, wound healing, Transwell and Colony formation assay. Protein expression analyzed via Western blot. In vivo antitumor efficacy assessed by xenograft study. Target expression in tumors confirmed by immunohistochemical staining. Statistical analysis used t-test/ANOVA (p<0.05).

Results

This study discovered that cancer associated fibroblasts (CAFs) in the tumor microenvironment induce Osimertinib resistance in NSCLC, and further study revealed that CA

SUBMITTER: Fan X 

PROVIDER: S-EPMC12283998 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature