Mesenchymal stem cell activity across a graded scaffold-hydrogel composite biomaterial for tendon-to-bone enthesis repair.

ABSTRACT: The severity of rotator cuff injury outcomes and a lack of tendon-to-bone enthesis regeneration strategies have inspired advances in biomaterials science to develop methods for interfacial tissue engineering. Here, we demonstrate a triphasic biomaterial comprising a non-mineralized, anisotropic collagen scaffold and a mineralized isotropic collagen scaffold linked via a continuous thiolated gelatin (Gel-SH) interface. This material provides a stratified environment in composition and porous architecture, and we report functional activity of human mesenchymal stem cells (hMSCs) across the scaffold. Notably, MSCs can be seeded onto the triphasic biomaterial and remain viable for up to 21 days. In addition, MSCs within the interfacial Gel-SH interfacial zone express markers associated with th

SUBMITTER: Timmer KB 

PROVIDER: S-EPMC12284061 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature