Correlative Imaging and super resolution microscopy studies reveal complexities in determining live-dead state of bacteria.

ABSTRACT: Imaging techniques are widely used to determine the physiological state of bacterial cells and provide an important platform for antibacterial evaluation in biofilms research. The commercial counter-staining SYTO 9 - propidium iodine kit is a popular choice for viability studies, with cell membrane damage due to antimicrobial action leading to replacement of the SYTO 9 dye with propidium iodine. This study investigates the live-dead state of cells in early-stage Staphylococcus aureus biofilms using correlative Fluorescence Microscopy (FM), Scanning Electron Microscopy (SEM) and super resolution Structural Illumination Microscopy (SIM). Correlative imaging data obtained at the single-cell level show that the physiological states of individual bacterial cells indicated by the SYTO 9 -

SUBMITTER: Luo J 

PROVIDER: S-EPMC12284284 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature