Myeloid TGF-β signaling shapes liver macrophage heterogeneity and metabolic liver disease pathogenesis.

ABSTRACT:

Background & aims

Cellular heterogeneity of innate immune cells, such as macrophages, in the liver is a hallmark of metabolic dysfunction-associated steatohepatitis (MASH) pathogenesis. However, the mechanisms shaping liver macrophage heterogeneity and function during disease progression remain poorly understood.

Methods

Control or myeloid-specific Tgfbr1 knockout mice (n = 9-12 per group) were fed a 12-week choline-deficient, amino acid-defined high-fat diet (CDA-HFD) or a 20-week GAN diet (40% fat, 22% fructose, 2% cholesterol). Liver tissue was analyzed using histopathology, quantitative PCR, immunoblotting, flow cytometry, and RNA sequencing (RNA-seq). Bulk RNA-seq (n = 3 per group) and single-nucleus RNA-seq were performed to investigate transcriptional reprogram

SUBMITTER: Meng Z 

PROVIDER: S-EPMC12284356 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature