The iron metalloproteome of Pseudomonas aeruginosa under oxic and anoxic conditions.

ABSTRACT: Pseudomonas aeruginosa is a major contributor to human infections and is widely distributed in the environment. Its ability for growth under aerobic and anaerobic conditions provides adaptability to environmental changes and in confronting immune responses. We applied native 2-dimensional metalloproteomics to P. aeruginosa to examine how use of iron within the metallome responds to oxic and anoxic conditions. Analyses revealed four iron peaks comprised of metalloproteins with synergistic functions, including (1) respiratory and metabolic enzymes, (2) oxidative stress response enzymes, (3) DNA synthesis and nitrogen assimilation enzymes, and (4) denitrification enzymes and related copper enzymes. Fe Peaks were larger under anoxic conditions, consistent with increased iron demand due to anae

SUBMITTER: Saito MA 

PROVIDER: S-EPMC12284477 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature