Proteins of the cancer cell secretome induce the protumoral microenvironment of diffuse intrinsic pontine glioma.

ABSTRACT:

Background

The microenvironment of diffuse intrinsic pontine glioma (DIPG) is devoid of infiltrating lymphocytes and immune checkpoint molecules, with the exception of B7-H3. Here, we studied whether the cancer secretome is a determinant of such tumor phenotype.

Methods

We quantified immune histologic markers in paraffin-embedded DIPG samples and healthy brainstem controls. We identified and quantified cytokines in frozen tissue samples, DIPG culture supernatants, cerebrospinal fluid (CSF) and plasma from patients and controls. We studied the phenotype of mesenchymal cells, brain microvascular endothelial cells and macrophages following their exposure to DIPG secretomes.

Results

We found profuse infiltration of anti-inflammatory CD163⁺ microglia/macrophages

SUBMITTER: Baulenas-Farres M 

PROVIDER: S-EPMC12284641 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature