HaloTag-Modified, Ferrocene Labeled Self-Assembled Monolayers for Protein Sensing.

ABSTRACT: Ferrocene (Fc)-based disulfide molecules of various lengths with amino acid scaffolds and alkane or oligo(phenylene-ethynylene) (OPE) bridges are used in a mixed SAM with a di-(ethylene oxide) terminal mercaptoundecanol diluent (PEG₂). The relative height of the Fc redox reporter in the SAM is compared to determine if there are protective effects like antifouling and specific detection. The HaloTag-binding motif is used as a proof-of-concept to investigate the electrochemical response to the HaloTag protein due to its known covalent and fast linkage. When the Fc-SAMs are exposed to the HaloTag protein, there are an antifouling nature and more specific detection for the engulfed Fc-based molecules (C₆tBu/Halo). The further out the Fc is from the SA

SUBMITTER: Brotherton AR 

PROVIDER: S-EPMC12284956 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature