Identification of circulating human papillomavirus types through high-throughput sequencing of Canadian municipal and institutional wastewater samples.

ABSTRACT: Human papillomaviruses (HPVs) are a diverse group of viruses that infect human mucosal and cutaneous tissues, with a ubiquitous global distribution. Several types are classified as high-risk due to their association with cervical, anal, penile, and oropharyngeal cancers. Given its widespread prevalence and the limitations of clinical surveillance, HPV is an ideal candidate for wastewater-based testing (WBT), as a way to characterize circulating HPV types. Toward this goal, we utilized high-throughput Illumina-based amplicon sequencing targeting the conserved GP5+/GP6+ sites of HPV in wastewater samples collected from two urban treatment plants, two town sites, and three correctional facilities. Larger population catchments displayed greater diversity of HPV types in wastewater compared to

SUBMITTER: Giesbrecht SJ 

PROVIDER: S-EPMC12285240 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature