Insights into genetic determinants of volatile fatty acid catabolism in <i>Cupriavidus necator</i> H16.

ABSTRACT: The soil bacterium Cupriavidus necator H16 is a promising host for upgrading waste-derived volatile fatty acids (VFAs) into renewable biochemicals. While bacterial VFA metabolic pathways are well understood, the C. necator genome encodes multiple enzymes for each catabolic step, and the degree of substrate specificity among these homologs is currently unknown. To gain insight into the catabolism of VFA substrates in C. necator, we performed transcriptomics on cells grown with acetate, propionate, butyrate, valerate, or hexanoate as the sole source of carbon and energy. These data revealed that C. necator upregulates multiple sets of genes putatively involved in substrate activation and β-oxidation in response to VFAs. To better understand this redundancy,

SUBMITTER: Holmes EC 

PROVIDER: S-EPMC12285253 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature