Ultrahigh-throughput screening of environmental bacteria for proteolytic activity using droplet-based microfluidics.

ABSTRACT: Exploration of diverse microbial sources, particularly environmental bacteria, is needed to identify novel and efficient peptidases/proteases that can be used in a variety of industrial applications. However, conventional function-based screening methods are inefficient and preclude the use of diverse microbial resources. This study illustrates a revolutionary approach to microbial screening using droplet-based microfluidics and fluorescence-activated droplet sorting that targets endopeptidases. Droplet-based microfluidic systems are a powerful tool for culturing microorganisms and for detecting microbial functions inside droplets with ultra-high throughput. However, droplet-based microfluidics for screening the proteolytic activity of environmental bacteria at a large scale remains largel

SUBMITTER: Nakamura A 

PROVIDER: S-EPMC12285256 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature