<i>Aeromonas</i> spp. as a fast-growing high-performance chassis for protein production.

ABSTRACT: Recombinant protein production is crucial for biotechnology and industrial processes. While Escherichia coli and other bacterial systems are effective, alternative systems can complement their limitations in specific applications. We introduce AMAX, a fast-growing high-performance bacterial chassis with target protein yields comprising 60-70% of total protein content. AMAX is compatible with common protein expression vectors, exhibits a growth rate comparable to Vibrio natriegens, and can adapt to diverse conditions, including co-production with E. coli, freshwater to seawater salinity, and contaminant phages. We also demonstrate the versatility of AMAX in producing several commercially valuable enzymes at high yield and purity. Transcriptomic and proteomic analyses hi

SUBMITTER: Tang M-X 

PROVIDER: S-EPMC12285260 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature