Bax- Bcl-xL interaction dynamics during the progression of cell cycle and cell death using FLIM-FRET.

ABSTRACT: Genetically identical cells in a population show cell-to-cell variability because of fluctuation in transcription, epigenetics, post-translational modifications, and stochastic or extrinsically triggered non-genetic alterations. The change in the interaction state of proteins also emerges as an additional layer of cell signaling that influences cell cycle and cell death. However, the interrelation between cell cycle progression and cell death under the influence of spatio-temporal changes in protein-protein interaction is difficult to demonstrate in growing cells. This requires tools for cell cycle phase-resolved visualization of macromolecular interactions in live cells. We describe an approach to visualize the interaction of pro- and anti-death signaling partners, Bax and Bcl-xL, during

SUBMITTER: Halikar AM 

PROVIDER: S-EPMC12285600 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature