Profiling miRNA changes in Epstein-Barr virus lytic infection identifies a function for BZLF1 in upregulating miRNAs from the DLK1-DIO3 locus.

ABSTRACT: Cellular and viral miRNAs are thought to play important roles in regulating Epstein-Barr virus (EBV) latent and lytic infections, however, to date, most studies have focussed on latent infections in B cells. To determine how cellular and viral miRNAs contribute to EBV lytic infection in epithelial cells, the main sites of lytic infection, we conducted miRNA-sequencing experiments in EBV-infected AGS gastric carcinoma cells, before and after reactivation to the lytic cycle, analysing both total miRNA and Ago2-associated miRNAs. We identified over 100 miRNAs whose association with Ago2 was affected upon EBV reactivation, most of which were due to changes in miRNA abundance. For EBV miRNAs, the most striking result was that the BHRF1 miRNAs, previously only reported to be expressed in B cells

SUBMITTER: Campbell AM 

PROVIDER: S-EPMC12286400 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature