High Glucose Promotes the Ferroptosis and Dysfunction of Endothelial Cells by Downregulating SLC3A2 and Promoting the Development of Nephropathy.

ABSTRACT: Background: Diabetic nephropathy, a leading cause of end-stage renal disease, is a major health concern. Its early-stage signs are unclear. Endothelial dysfunction, an early indicator, is suitable for early detection and intervention. However, current treatments mainly focus on glycemic and blood pressure control, lacking specific methods for targeting this dysfunction. Method: We reanalyzed GSE13535 dataset, which has single-cell RNA-seq of high-glucose-exposed endothelial cells. HUVECs were cultured in high-glucose and TNF-α. We conducted RNA extraction, qPCR, western blotting, iron measurement, TUNEL assay, and bioinformatics analysis. An antiferroptosis drug was used in STZ-treated diabetic mice. Results: Single-cell RNA-seq showed early endothelial cell dysfunctio

SUBMITTER: Ji Y 

PROVIDER: S-EPMC12286665 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature