Old mitochondria regulate niche renewal via α-ketoglutarate metabolism in stem cells.

ABSTRACT: Cellular metabolism is a key regulator of cell fate¹, raising the possibility that the recently discovered metabolic heterogeneity between newly synthesized and chronologically old organelles may affect stem cell fate in tissues^2,3. In the small intestine, intestinal stem cells (ISCs)⁴ produce metabolically distinct progeny⁵, including their Paneth cell (PC) niche⁶. Here we show that asymmetric cell division of mouse ISCs generates a subset enriched for old mitochondria (ISC^mito-O), which are metabolically distinct, and form organoids independently of niche because of their ability to recreate the PC niche. ISC^mito-O mitochondria produce more α-ketoglutarate, driving ten-eleven translocation-mediated epigenetic ch

SUBMITTER: Andersson S 

PROVIDER: S-EPMC12286850 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature