Naturally ornate RNA-only complexes revealed by cryo-EM.

ABSTRACT: The structures of natural RNAs remain poorly characterized and may hold numerous surprises^1-4. Here we report three-dimensional structures of three large ornate bacterial RNAs using cryo-electron microscopy (cryo-EM). GOLLD (Giant, Ornate, Lake- and Lactobacillales-Derived), ROOL (Rumen-Originating, Ornate, Large) and OLE (Ornate Large Extremophilic) RNAs form homo-oligomeric complexes whose stoichiometries are retained at lower concentrations than measured in cells. OLE RNA forms a dimeric complex with long co-axial pipes spanning two monomers. Both GOLLD and ROOL form distinct RNA-only multimeric nanocages with diameters larger than the ribosome, each empty except for a disordered loop. Extensive intramolecular and intermolecular A-minor interactions, kissing loops, an unusual

SUBMITTER: Kretsch RC 

PROVIDER: S-EPMC12286853 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature