Optimized workflow for behavior-coupled fiber photometry experiment: improved data navigation and accessibility.

ABSTRACT: Fiber photometry provides crucial insights into cell population activity underlying behavior. While numerous open-source data analysis tools exist, few offer an automated workflow that streamlines the analysis of fiber photometry data alongside behavioral measurements, by enabling more intuitive and facilitated navigation within data files. We developed here a workflow starting from the intracerebral implantation of optical fibers in mice, to the analysis of fiber photometry signals, aligned with recorded behavioral data. This tool is particularly valuable for studying unpredictable exploratory behaviors, as it allows efficient and rapid revisiting of fiber photometry signals aligned to spontaneous behavioral changes. Our approach allows ease of data analysis and exploration using custom a

SUBMITTER: Athanassi A 

PROVIDER: S-EPMC12287066 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature