Transcriptomic and protein-protein interaction network analyses of the molecular mechanisms underlying the mycorrhizal interaction in <i>Cypripedium macranthos</i> var. <i>rebunense</i>.

ABSTRACT:

Introduction

Orchid mycorrhizal (OM) symbiosis plays an essential role in orchid seed germination and development, but its molecular mechanisms remain largely unexplored.

Methods

To comprehensively analyze gene expression associated with early fungal colonization, transcriptome analysis of Cypripedium macranthos var. rebunense was performed using mycorrhizal tissues prepared by inoculating seedling plants with a fungus that exhibited different mycorrhizal interaction properties among subcultures.

Results

Colonization with the mycorrhizal fungus induced an increased expression of orchid genes encoding enzymes involved in cell wall synthesis, degradation, and modification, as well as those encoding transporters of sugars, amino acids, nucleic acids, and other nit

SUBMITTER: Jin C 

PROVIDER: S-EPMC12287105 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature