Single cell ICP-MS for the assessment of potential nephroprotectors against cisplatin.

ABSTRACT: The use of cisplatin chemotherapy is often limited by the occurrence of various side effects, with renal toxicity being one of the most serious. In the present work, a single cell ICP-MS (scICP-MS) methodology was optimised to evaluate the cellular uptake of cisplatin in the presence of three potential nephroprotectors such as chitosan-stabilised selenium nanoparticles (Ch-SeNPs), selenomethionine (SeMet) and methionine (Met). Human telomerase reverse transcriptase-immortalised renal proximal tubular epithelial cells (RPTEC/TERT1) and human cervical cancer cells (HeLa) were employed with this aim. In both cell lines, a decrease in the intracellular Pt levels when using SeMet and Met as coadjuvants was revealed, involving less toxicity in renal cells but no reduction in the anticancer effec

SUBMITTER: Iglesias-Jimenez A 

PROVIDER: S-EPMC12287176 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature