Optimization of selection agent concentrations and expanding G418 utility for gentamicin resistance in Marchantia polymorpha.

ABSTRACT: Genetic transformation of plants is pivotal for advancing biotechnology, with success depending largely on effective selection methods. Marchantia polymorpha has emerged as a model plant due to its evolutionary importance, ease of manipulation, and simple genetic structure. However, inconsistent antibiotic performance and limited studies on optimal selection agent concentrations have posed challenges. This study aimed to optimize selection agent use in M. polymorpha genetic transformation. We assessed the effects of five antibiotics (hygromycin, kanamycin, G418, neomycin, and gentamicin) and the herbicide chlorsulfuron on M. polymorpha gemmae growth by evaluating survival, morphological properties, area, and fresh weight of the thallus on these selection media. For each agent, we identifie

SUBMITTER: Poormassalehgoo A 

PROVIDER: S-EPMC12287465 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature