Exploring the inhibitory effect and mechanism of 3,4,5-trihydroxybiphenyl on α-glucosidase: an integrated experimental and computational approach.

ABSTRACT: 3,4,5-Trihydroxybiphenyl (THB) is a naturally occurring compound derived from Sorbus pohuashanensis, primarily reported for its antifungal activity. However, its potential to inhibit α-glucosidase remains unclear. In this study, we assessed the inhibitory effects of THB on α-glucosidase and explored the mechanism of inhibition through kinetic analysis, multispectral techniques, molecular docking and molecular dynamics simulations. Furthermore, a sucrose tolerance test was performed to evaluate the effects of THB on postprandial blood glucose (PBG) levels in mice. The results showed that THB exhibited a non-competitive and reversible inhibitory effect on α-glucosidase, with IC₅₀, K_m, and K_i values of 11.52 μM, 0.69 ± 0.02 mM, and 26.26 ± 4.95 μM, resp

SUBMITTER: Guo R 

PROVIDER: S-EPMC12287757 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature